Developed validation for simultaneous determination of three Di-caffeoylquinic acid derivatives from the Leaf of Eribotrya japonica Lindl. by HPLC-DAD
نویسندگان
چکیده
The aim of this study was to developed HPLC simultaneous analysis method for the determination Caffeoylquinic acid isolated from leaf Eribotrya japonica Lindl. extract. phenolic compounds in E. leaves were and identified by HPLC, NMR, Mass etc. quantitative carried out HPLC-DAD using C18 column with gradient elution water acetonitrile at 280 nM 60 min. successfully validated specificity, LOD (linearity, limit detection), LOQ (limit quantification), precision recovery. three including 5-Caffeoylquinic (5-CQA), 4-Caffeoylquinic (4-CQA) 3-Caffeoylquinic (3-CQA). In a 3-CQA 7.82-14.48 µg/mL, 4-CQA 3.75-12.58 µg/mL 5-CQA 3.60-7.82 standard chemicals, respectively. validation would be applicable extracts.
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ژورنال
عنوان ژورنال: Food Science and Technology
سال: 2021
ISSN: ['2331-513X', '2331-5156']
DOI: https://doi.org/10.1590/fst.68121